RSpak DE-413 的填料是一个步骤生成的，而ODS色谱柱的填料需要2个步骤，即先制造基质再键合上C18基团。简单的生产流程使DE系列色谱柱有很好的批号再现性。
可滚动
| Lot A | Lot B | Lot C | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| No. | Rt (min) |
Peak area (UV x sec) |
Peak area (%) |
No. | Rt (min) |
Peak area (UV x sec) |
Peak area (%) |
No. | Rt (min) |
Peak area (UV x sec) |
Peak area (%) |
| 1 | 2.50 | 36600 | 20.1 | 1 | 2.50 | 37600 | 20.1 | 1 | 2.48 | 36400 | 20.2 |
| 2 | 3.15 | 32200 | 17.7 | 2 | 3.15 | 33200 | 17.7 | 2 | 3.12 | 31900 | 17.7 |
| 3 | 4.88 | 48600 | 26.7 | 3 | 4.89 | 50100 | 26.7 | 3 | 4.85 | 48100 | 26.6 |
| 4 | 7.96 | 64800 | 35.6 | 4 | 8.00 | 66700 | 35.6 | 4 | 7.92 | 64300 | 35.6 |
Sample: 5 µL
- 1.0.2 % Methyl ethyl ketone, 2-Butanone
- 2.0.2 % Diethyl ketone, 3-Pentanone
- 3.0.4 % Di-n-propyl ketone, 4-Heptanone
- 4.0.6 % Di-n-butyl ketone, 5-Nonanone
- Column
- ：Shodex RSpak DE-413 (4.6 mm I.D. x 150 mm)
- Eluent
- ：CH3CN/H2O=50/50
- Flow rate
- ：1.0 mL/min
- Detector
- ：UV (262 nm)
- Column temp.
- ：40 ℃